Faculty Profile

Mr. Kundan Kumar Chaubey
Senior Research Officer
Institute of Applied Science & Humanities

Contact Details:

Email: kundan.chaubey@gla.ac.in
Contact Number: 9627740472

OBJECTIVE

To pursue a research-oriented career in biological science with my extensive knowledge and development & research skills. 

PERSONAL INFORMATION

Name                                  Kundan Kumar Chaubey

Father’s Name                    Awadhesh Kumar Chaubey

Date of birth                        03.01.1984

Sex                                     Male

Citizenship                         Indian

Marital Status                     Married

Language Known               English, Hindi, Bengali

Permanent Address           Vill+Post-Bherokhara, Via-Tajpur, District-Samastipur, Bihar, India 848130

Contact No.                         +91-9334171964, +91-7488261125

Email                                   kundan1984chaubey@yahoo.co.in

 EDUCATIONAL QUALIFICATION

Sl. No

Name of Examination

Board/Univ.

Year of passing

Subjects

%/CGPA

1

Ph.D.

(Microbiology)

GLA University, Mathura

Pursuing

(Continuing since  July, 2013)

Research methodology, bioinformatics & Instrumentation, Chemical biology

7.33

(CGPA)

Ph.D. course

work

2

M.Sc

(Microbiology)

Bundelkhand University, Jhansi

2007

 

Microbiology

71.6%

3

B. Sc.(Industrial Microbiology)

B.R.A. B.U., Muzaffarpur, Bihar

2005

Industrial Microbiology, Botany & Chemistry

73.4%

4

Intermediate

B.I.E.C., Patna

2000

A/c to Board

69.3%

5

High School

B.S.E.B., Patna

1998

A/c to Board

54.4%

Computer and other qualification:

  • One year Computer higher diploma
  • One year type writing diploma

AWARDS:

  • GATE-2010 score card
  • Obtained first prize in poster presentation in ISSGPU-2012
  • ‘Helping Hand Award’ (Sum of USD 1000), free registration and 2 years membership of IAP, USA awarded by International Association for Paratuberculosis (IAP) for attending the 13th International Colloquium on Paratuberculosis (ICP) 2016, will beheld in Nantes, France (20 – 24 June 2016)
  • Obtained second prize in Oral presentation in first colloquium on Mycobacterial diseases: International status & Priorities at Amity University of Rajasthan, Jaipur, 12-13 April, 2016.
  • Obtained second prize in Oral presentation in first colloquium on Mycobacterial diseases: International status & Priorities at Amity University of Rajasthan, Jaipur, 12-13 April, 2016.
  • ‘NRDC Societal Innovation award, 2014’ for innovationentitled‘Indigenous vaccine against Johne’s Disease in domestic livestock’ awarded in 2016.
  • ‘Certificate of Honour’ for the Best Research article published in Intas Polivet,15 (1), 2016. 

RESEARCH EXPERIENCE 

(A). As a Ph.D. Student at GLA University, Mathura and Central Institute for Research on Goats, Farah, Mathura (Continuing since, July2013) (5 years 1 Month). 

  • MHC-I Binding Predictions (IEDB analysis resource) for MAP CFPs using BOLA-T7 allele by Netmhcpan method.
  • Characterization of 6 MAP CFPs proteins using Raptorx software
  • Immuno-proteomic analysis of recombinant secretory proteins has done (SDS-PAGE analysis, Protein estimation and Western blot analysis for Immuno-reactivity of proteins)
  • Recombinant CFPs based individual/cocktail ELISA have evaluated as a diagnostic marker with Indigenous ELISA kit in different domestic livestock species (Goats, sheep, cattle and buffaloes) is under process.
  • Optimization, laboratory scale and field validation of DIVA ELISA.
  • Species wise sero-reactivity and immuno-dominancy will be evaluated in individual secretory protein by western blot and plate ELISA and cocktail of secretory proteins will be evaluated by plate ELISA only.
  • Comparison of indigenous ELISA tests based on semi-purified protoplasmic antigens and recombinant secretary proteins for the diagnosis of spontaneous cases of Mycobacterium avium subspecies paratuberculosis infection in domestic ruminants and human beings.
  • Comparative study of c_ELISA, i_ELISA and Microscopy with respect to histo-pathological lesions in slaughtered and necropsied goats and slaughtered buffaloes naturally endemic for Mycobacterium avium subsp. paratuberculosis infection
  • Molecular diversity and homology in six CFPs genes in novel bio-type of Mycobacterium avium subspecies paratuberculosis of goat origin MAP ‘S 5’ Indian Bison type versus other strains
  • Cell mediated immunity and Rat spleenocytes cytotoxicity against six MAP CFPs.
  • Study of immunogenicity of CFPs in Immunized rabbits by c_ELISA, i_ELISA
  • GPX (Glutathione peroxidase) and IL-10 gene expression in MAP secretome treated Rat spleenocytes 
  • IL-10  gene expression in MAP secretome treated Rabbits.

(B). At Central Institute for Research on Goats, Farah, Mathura as a Research Assistant  SinceJune, 2015 (3 years 1 Month)

  • Study the association of Mycobacterium avium subspecies paratuberculosis with Crohn’s Disease
  • Study microbiome, transcriptome and immune interactions in Crohn’s disease and Johne’s disease with reference to MAP infection.
  • Genetic finger printing and comparative genomics of human and animal MAP isolates.
  • Correlation of disease behaviour and treatment outcomes inulcero-constrictive ileocolonic disease (intestinal tuberculosis and Crohn’s disease) with and without MAP infection
  • Incidence and Prevalence of Crohn’s Disease
  • Screening of samples (blood, serum, fecal) from farms of domestic livestockand screened by multiple diagnostic tests (microscopy, ELISA, PCR, culture).
  • Screening of samples (tissues, blood, serum and fecal) from slaughtered and necropsied animals using multiple diagnostic tests (microscopy, ELISA, PCR, histo-pathological sections by H&E staining).
  • Screening of tissues from slaughtered and necropsied animals by Immuno Histochemistry (IHC).
  • Tissue samples fromdomestic livestock found negative, suspected and positive for MAP infection  by multiple tests (microscopy, ELISA, PCR) and processed for screening by In-situ PCR.
  • Sub-culture of MAP isolates and genetic finger printing (IS1311 PCR REA, gyrB PCR-REA, IS1311 L2 PCR-REA ).
  • Short Sequence Repeat (SSR) typing of Mycobacterium aviumsubspecies paratuberculosisisolates using Locus 1 mono-nucleotide G repeat, Locus 2 mono-nucleotide G repeat (10G Repeat), Locus 8tri-nucleotide GGT repeat, Locus 9tri-nucleotide TGC repeat
  • Sub-typing/ Bio-typing: MIRU-VNTR typing of Mycobacterium aviumsubspecies paratuberculosisisolates using VNTR X3, VNTR3, VNTR7, VNTR10, VNTR25, VNTR32, VNTR47, VNTR292, MIRU 1, MIRU 4, VNTR259, VNTR1067.
  • Screening of commercial milk and milk products by multiple diagnostic tests (microscopy, ELISA, PCR, culture, LAT, dotELISA, FAT).
  • New isolates of MAP from samples originating from different sources were identified and confirmed and their glycerol stocks were prepared. 

(C). At Central Institute for Research on Goats, Farah, Mathura
January 2012 to Dec. 2014 (as a Research Associate): 3 years on Mycobacterium avium subspecies paratuberculosis (MAP) (cause of Johne’s disease)

  • Isolation and identification of MAP from clinical specimens by using techniques such as Micoscopy, culture, PCR, qRT-PCR, ELISA, Immunofluorescence, LTT, LAT, FAT, Dot-ELISA etc.
  • Preparation of Johne’s disease vaccine, Vaccination, and evaluation of antibody and cell mediated immune response against MAP in animals (sheep, goats, cattle, buffaloes).
  • Development of Johne’s disease Vaccine.
  • Evaluation of effect of vaccine measured by shedding of MAP in feces by microscopy and qRT-PCR and presence of MAP in blood by PCR and qRT-PCR.
  • Isolation and phylogenetic analysis of an orf virus.
  • Isolation, identification and characterization of a Peste des Petits Ruminants virus
  • Isolation and Identification of a goat pox virus
  • Role of Receptor tyrosine kinase in regulation of  Peste des Petits Ruminants virus replication
  • Role of Silver nanoparticles in impairment of Peste des petits ruminants virus replication 

(D)Teaching Experience:11 months (Feb 2011 to Jan 2012) experienceas a Post Graduate Teacher (PGT) Biology fromDon & Donna Convent, Shahjahanpur, Uttar Pradesh, India 

(E) At Department of Genetics, Ballygunj Science College, Calcutta University: 29 months (Jan, 2009 to Feb 2011) as a honorary Fellow and JRF on“Study of mitochondrial dysfunction in response to HIV infection and its treatment”. 

  • Maintenance of adherent and non-adherent animal cell lines culture (293-T cells, CEM cells, GHOST cells).
  • Viable cell count using trypan blue stain through hemocytometer.
  • Mitochondria isolation from adherent and non-adherent cells.
  • Mitochondrial DNA, RNA, Protein isolation from adherent and non adherent cell lines.
  • Virus production by Calcium phosphate transfection method.
  • Virus purification by sucrose gradient ultracentrifugation method.
  • Measurement of GFP expression in GHOST cells during HIV infection.
  • HIV infected and ART treated total cell proteome and mitochondrial proteome analysis by SDS-PAGE.
  • HIV infected and ART treated total cell proteome and mitochondrial proteome analysis by 2D Gel Electrophoresis.
  • Primer designing for specific mitochondrial gene.
  • PCR for specific mitochondrial gene from mitochondrial DNA.
  • MTT assay for measurement of cell viability in HIV infected and ART treated.
  • Primers designing for HIV specific Gene.
  • HIV specific RT-PCR from total mitochondrial RNA for confirmation of HIV transcript inside mitochondria. 

(F). At Banaras Hindu University (Oct 2008 to January 2009) (As a Research Scholar): 4 months

          (a). On Study of mitochondrial dysfunction in response to HIV infection  and its treatment”.

  • Preparation and of HIV pseudotyped viruses (calcium phosphate transfection) and evaluation of antiviral activity.
  • Study on genome-wide expression (upregulation/downregulation) and total cell/mitochondrial proteome in response to ART/HIV infection (SDS-PAGE, 2D Gel Electrophoresis).
  • Study on HIV transcript integration in mitochondrial genome (Mitochondria, mt-DNA, mt-RNA, RT-PCR, nested PCR).

(G). At Indian Institute of Chemical Biology (IICB), Kolkata, India (6th March to 31st August 2007) (as a trainee)

          (a).  On Cloning and expression of putative protein of P. falciparum

  • Cloning and Overexpression of pfARP (Parasite culture, Isolation from infected erythrocytes, extraction of RNA, C-DNA synthesis, PCR amplification, Cloning, over-expression, purification, antibody generation, Western blotting).
  • Evaluation of stage specific expression of PfARP and Subcellular localization (Semiquantitative RT-PCR , Immune fluorescence microscopy).

 (H). At Central Drug Reasearch Institute (CDRI), Lucknow, India. (From 13th June to 12th October, 2006)(as a trainee)

         (a).  On Antioxidant and Anti-apoptotic effect of melatonin to protect the liver from Plasmodium yoeli  induced apoptosis

  • Study the liver of mice during malaria infection (In vivo maintenance of Plasmodium yoeli) and protective effect of melatonin.
  • Evaluation of anti-oxidant and anti-apoptotic activity of melatonin (Reduced glutathione, Protein carbonyl, lipid peroxidation, OH radical (OH) and Caspase 3 Assay).

(I) TRAINING AND OTHER PROJECTS  (During B.Sc: 2001-2005)

  • Dairy Microbiology, Vaccine production, Bakes production, Beverage production, Water & Sewage Treatment. 

Publications:

  1. K.K. Chaubey, S.V. Singh, A.K. Bhatia, Evaluation of ‘Recombinant secretary antigens’ based ‘Cocktail ELISA’ for the diagnosis of Johne's disease and to differentiate non-infected, infected and vaccinated goats in combination with indigenous ELISA test, Small Rumin. Res. 165 (2018) 24-29. https://doi.org/10.1016/j.smallrumres.2018.06.005.
  2. K.K. Chaubey, S.V. Singh, A.K. Bhatia, Comparison of ‘Indigenous ELISA kit’ with ‘Ethanol Vortex ELISA kit (USA)’ for the detection of Mycobacterium avium subsp. paratuberculosis infection in cattle population in ‘Reverse Ice-burg’ environment: In search of a ‘globally relevant kit’, Indian J. Exp. Biol. 56 (2018) 279-286.
  3. Chaubey KK, Kumaresan G, Gupta RD, Singh SV, Bhatia AK, et al. (2018) Molecular Diversity and Homology in Six CFPs Genes in the Novel Bio-Type, 'Indian Bison Type' of Mycobacterium avium subspecies paratuberculosis of Goat Origin vs. other Biotypes. Mycobact Dis 8: 267. doi:10.4172/2161-1068.1000267
  4. Chaubey KK, Singh SV, Bhatia AK, Gupta RD, Gupta S, et al. (2018) Detection Limits of Recombinant Secretary Proteins versus Semipurified Protoplasmic Antigens for the Diagnosis of Spontaneous Cases of Mycobacterium avium subspecies paratuberculosis Infection in Domestic Ruminants. J Vet Sci Technol 9: 552. doi:10.4172/2157-7579.1000552.
  5. Chaubey KK,  Gupta RD,  Gupta S, Singh S.V, Bhatia AK, Jayaraman S, Kumar N, Goel A, Rathore AS, Sahzad, Sohal JS, BJ, Singh M, Goyal M, Dhama K, Derakhshandeh A. (2016). Trends and advances in the diagnosis and control of paratuberculosis in domestic livestock. Veterinary Quarterly, 36 (4): 203-227.
  6. Chaubey KK, Singh S.V., Gupta S, Singh M, Sohal JS, Kumar N, Singh MK, Bhatia AK, Dhama K. (2017). Mycobacterium avium subsp. paratuberculosis - an important food borne pathogen of high public health significance with special reference to India: an update. Veterinary Quarterly. 37 (1): 282-299. DOI:10.1080/01652176.2017.1397301.
  7. Chaubey KK, Singh SV, Gupta S, Jayaraman S, Singh M, Stephan BJ, Rawat KD, Pachoori A, Dhama K & Bhatia AK. 2015. Diagnostic potential of three antigens from geographically different regions of the world for the diagnosis of Ovine Johne’s disease in India. Asian Journal of Animal and Veterinary Advances. 10 (10): 567-576, 2015.
  8. Kundan Kumar Chaubey, Shoor Vir Singh, Ashok Kumar Bhatia. 2018. Evaluation of ‘Cocktail ELISA’ developed from ‘recombinant secretory proteins’ of Mycobacterium avium subsp. paratuberculosis with ‘Indigenous ELISA kit’ for the detection of infected and vaccinated animals (DIVA). Indian Journal of experimental Biology (Under Review).
  9. Kundan Kumar Chaubey, Rinkoo Devi Gupta, Shoor Vir Singh, Ashok Kumar Bhatia, Naveen Kumar, Abhishek Singh Rathore, Saurabh Gupta, Manju Singh, Kuldeep Dhama. 2018. Cloning and expression of six cultural filtrate proteins from novel native 'Indian Bison type' of Mycobacterium avium subspecies paratuberculosis ‘S 5’ of goat origin and their evaluation for sero-diagnosis Johne's disease in endemically infected goats and cattle in India. Protein Expression and Purification (Under Review).
  10. Singh S.V, Kuenstner JT, Davis WC, Agarwal P, Kumar N, Singh D, Gupta S, Chaubey KK, Tyagi AK, Misri J, Jayaraman S, Sohal JS and Dhama K. (2016). Concurrent resolution of chronic diarrhea likely due to Crohn's disease and infection with Mycobacterium avium paratuberculosis. Front. Med. 3:49. http://dx.doi.org/10.3389/fmed.2016.00049.
  11. Audarya SD, Singh M, Stephen BJ, Sahzad, Chaubey KK, Gupta S, Pachoori A, Jayaraman S, Aseri GK, Sohal JS, Bhatia AK, Singh SV. 2018. Status of Johne’s disease in buffalo population of Malwa region using goat based indigenous ELISA kit. Buffalo Bulletin. 37: 9-16.
  12. Stephen B.J., Singh M., Singh S.V., Gupta S., Chaubey K.K., Sahzad, Jayaraman S., Jain M., Sohal J.S., Dhama K.         (2016). Estimation of rate of 'Bio-contamination' and ‘bio-typing’ of Mycobacterium avium subsp. paratuberculosis in fresh cottage cheese (Paneer) sold by the local dairy shops in rural, semi-urban and peri-urban regions of South Uttar Pradesh of North India using multiple diagnostic tests. Advances in Animal and Veterinary Sciences, 4 (8): 441-448.
  13. Singh S.V., Stephen B.J., Singh M., Sohal J.S., Gupta S., Chaubey K.K., Sahzad, Jayaraman S., Sachan T.K., Aseri G.K., Jain M, Datta M., Dhama K. (2016). Evaluation of indirect flurorescent antibody test as potential screening test’ for Mycobacterium avium subsp. paratuberculosis infection in the milk lactating domestic livestock.               Journal of Experimental and agricultural Sciences, 4(5): 533-540.
  14. Sahzad, Gupta S, Chaubey KK, Jayaraman S, Singh M, Stephan BJ, Deendayal, Jain M, Pachoori A, Jagdip Singh Sohal, Bhatia AK, Singh S.V. (2016). Comparative evaluation of two screening tests (serum ELISA and fecal microscopy) for the estimation of Johne’s disease infection in goatherds endemically infected with Johne’s disease.              Journal of Ruminant Science. 4: 29-32.
  15. Audarya, S.D., D. Chhabra, Sahzad, M. Singh, BJ Stephan, KK Chaubey, S Gupta, JS Sohal, K Dhama, SY Mukartal, D Rathnamma, SV Singh. (2016). Bio-incidence of bovine Johne’s disease in dairy buffaloes in Central and North India using sensitive goat based ‘indigenous ELISA kit’ and traditional tests. J. Exp. Biol. Agric. Sci., 4: 525-532.
  16. Singh S.V., Stephen B.J., Singh M., Gupta S., Chaubey K.K., Sahzad., Jayaraman S., Aseri G.K., Sohal J.S., Bhatia A.K., Pachoori A., Chauhan C. and Dhama K. (2016). Evaluation of milk dot ELISA as ‘field based test’ vis a vis milk plate ELISA for the detection of Mycobacterium avium subsp. paratuberculosis infection in lactating domestic livestock. Indian Journal of Biotechnology, 15(2): 166-171.
  17. Singh, S.V., Stephen, B.J., Singh, M., Gupta, S., Chaubey, K.K., Sahzad, Sachan T.K., Sohal, J.S., Dhama, K., Mukartal S.Y., Hemati Z. (2016). Comparison of newly standardized ‘Latex milk agglutination test’, with ‘Indigenous milk ELISA’ for ‘on spot’ screening of domestic livestock against Mycobacterium avium subsp. paratuberculosis infection. Indian J of Biotechnology 15: 511-517.
  18. Gupta S., Singh S.V., Gururaj K., Chaubey K.K., Singh M., B. Lahiri, Agarwal P., Kumar A., Misri J and Sohal J.S.         (2017). Comparative evaluation of traditional IS900 PCR assay with newer assays (‘Taqman probe PCR’ and ‘SYBR green Real time PCR’) in thyroid disorder patients sero-positive for Mycobacterium avium subsp. paratuberculosis using Indigenous ELISA kit. Indian Journal of Biotechnology. 16 (2):228-234
  19. Singh S.V, Gupta S, Chaubey K.K, Bhushan S, Rawat K.D, Kumar N, Tiwari HA, Chaturvedi V, Sohal J.S, Dhama K and Hemati Z. (2017). ‘Therapeutic Management’ of Incurable Paratuberculosis Using ‘Indigenous Vaccine’ in Goatherds, Endemically Infected with Johne’s Disease. International Journal of Pharmacology, 13: 145-155
  20. Mukartal S.Y, Rathannama D, Narayanswamy H.D, Gupta S, Chaubey K.K, Singh M, Hemati Z, Nishanth C, Pachoori A, Dhama K, Singh S.V. (2017). Assessment of Ovine Johne’s disease in the Mandya sheep breed in South India using multiple diagnostic tests and bio-typing of Mycobacterium avium subsp paratuberculosis infection. Cogent Food & Agriculture (2017), 3: 1298391.
  21. Chaturvedi, Shivam, Singh, SV, Srivastava, A, Gangwar, Neeraj, Kumar, Naveen, Rawat, K.D., Gupta, S, Chaubey, K.K., Singh, Ravpreet, Dhama, K. (2017). Comparative evaluation of FAT, IS900 PCR and microscopy vis-a-vis histopathology for the detection of Mycobacterium avium subsp paratuberculosis infection in tissues of goats naturally died in herds endemic for Johne's disease. Indian Journal of Animal Sciences. 87. 685-693.
  22. Sahzad, Singh SV, Gupta S, Chaubey KK, Singh M, Dayal, Deen, Dhama K, Hemati, Z, Srivastava M, Mansi. (2017).        Comparison of Antibody Response and Direct Microscopy between Male and Female Goats in Response to Infection of Mycobacterium avium subsp. paratuberculosis in Goatherds Endemic for Johne’s Disease. JSM Microbiology. 5. 1034.
  23. Sahzad, Singh SV, Chaubey KK, Gupta S, Kumar, P, Kumar R, Pratap Singh, Akhand. (2017). A New Rapid and Cost Effective Genomic DNA Extraction Method for Molecular Diagnosis of Mycobacterium avium subsp. paratuberculosis from Various Sources. JSM Microbiology. 5. 1036.
  24. Manju Singh, Shoor Vir Singh, Saurabh Gupta, Kundan Kumar Chaubey, Manoj Kumar Singh, Bjorn John Stephan, Jagdip Singh Sohal, Kuldeep Dhama, Zahra Hemati. (2017). Comparative evaluation of multiple antigen and antibody based tests for estimating bio-load and bio-type profile of Mycobacterium avium  subsp.  paratuberculosis in raw milk of goats endemically infected with Johne's disease. Tropical Animal Health and Production (Under Review).
  25. Manju Singh, Saurabh Gupta, Shoor Vir Singh, Kundan Kumar Chaubey, Jagdip Singh Sohal, Kuldeep Dhama. (2017). Bio-incidence of   Mycobacterium subspecies  paratuberculosis in the pasteurized liquid milk, flavoured milk and milk powder commercially sold by leading market brands in North India              Journal of Experimental Biology and Agricultural Sciences (Accepted and Revised).
  26. Manju Singh, Shoor Vir Singh, Saurabh Gupta, Kundan Kumar Chaubey, Bjorn  John Stephan, Jagdip Singh Sohal, Manali Dutta. (2017). A novel ‘Nano-immuno test’ for the detection of live Mycobacterium avium subsp. paratuberculosis using magnetic nano-particles and chromogen. Veterinary Research Communications (Submitted).
  27. Sharma, S, Singh, S.V., Sangwan, V., Singh, V., Chaubey, K.  K., Gupta, S, Singh, M. (2017). Metabolic adjusftments as signatures for pathogenesis and diagnosis of Johne’s disease in goats. Haryana Vet (Accepted)
  28. Zahra, H., Dera-Khshandeh, A., Masoud, H., Singh, S. V., Chaubey, K.K. (2017). Novel recombinant Mce-truncated protein antigens for the diagnosis of Mycobacterium avium subsp. paratuberculosis infection in domestic livestock and control by developing a sub-unit vaccine. Res. in Vet Sci. (Communicated)
  29. Adil Majid Bhat, HU Malik, SV Singh, Tufail Hussain, KK Chaubhey, MS Mir, Sabia Qureshi, ZA Kashoo, Showkat-ul-Nabi, Manzoor-ur-Rehman, Rather Waseem Yousuf, Syed Ishfaq Qadri. (2017).     Bio-prevalence and molecular diagnosis of Mycobacterium avium subsp paratuberculosis infection in small ruminant population of Ganderbal district of Kashmir valley. Journal of Entomology and Zoology Studies 2018; 6(1): 01-04
  30. Sujata Jayaraman,  Mukta Jain, Kuldeep Dhama, S. V. Singh, Manali
    Datta, Neelam Jain, K.K.Chaubey, S. Gupta, G. K. Aseri, Neeraj Khare,
    Parul Yadav, A. K. Bhatia, J. S. Sohal. 2016. DIVA Technology:
    Indispensable Tool for the Control of Johne’s disease. Journal of
    Experimental Biology and Agricultural Sciences. 4(16-25).
  31. Singh SV, Kuenstner JT, Davis WC, Agarwal P, Kumar N, Singh D, Gupta S, Chaubey KK, Tyagi AK, Misri J, Jayaraman S, Sohal JS and Dhama K.2016. Concurrent cure of Crohn’s disease and infection with Mycobacterium avium paratuberculosis. Front. Med. 3:47. doi:10.3389/fmed.2016.00047.
  32. Shoor Vir Singh, Bjorn John Stephen, Manju Singh, Saurabh Gupta, Kundan Kumar Chaubey, Sahzad, Sujata Jayaraman, Tarun Kumar Sachan, Gajendra Kumar Aseri, ManaliDutta, Jagdip Singh Sohal, Kuldeep Dhama, Shivalingappa Yamanappa Mukartal and Doddamane Rathnamma. 2016. Evalution of “indirect fluorescent antibody test” as potential Screening test for mycobacterium avium subspecies paratuberculosis using milk of lactating domestic livestock. Journal of Experimental Biology and Agricultural Sciences. 4(5): 533-544.
  33. Sachin Digambar Audarya, Daljeet Chhabra, Sahzad, Manju Singh, Bjorn John Stephen, Kundan Kumar Chaubey, Saurabh Gupta, Jagdip Singh Sohal, Kuldeep Dhama, Shivalingappa Yamanappa Mukartal, Doddamane Rathnamma and Shoor Vir Singh. 2016. Bio-incidence of bovine Johne’s disease in dairy buffaloes in central and north india using sensitive goat based “Indigenous Elisa kit” and traditional tests. Journal of Experimental Biology and Agricultural Sciences. 4(5): 525-532.
  34. Shoor Vir Singh, Bjorn John Stephen, Manju Singh, Saurabh Gupta, Kundan Kumar Chaubey, Sahzad, Sujata Jayaraman, Gajendra Kumar Aseri, Jagdip Singh Sohal, Ashok Kumar Bhatia, Anjali Pachoori, Jitendra Chauhan and Kuldeep Dhama. 2016. Evaluation of new ‘indigenous milk dot-ELISA’ as ‘Field Test’ vis-à-vis milk plate-ELISA for the detection of Mycobacterium avium sub species paratuberculosis infection in lactating domestic livestock. Indian Journal of Biotechnology.15: 166-171
  35. Kumar NBarua SRiyesh TChaubey KK, Rawat KDKhandelwal NMishra AKSharma NChandel SSSharma SSingh MKSharma DK, Singh SVTripathi BN. Complexities in Isolation and Purification of Multiple Viruses from Mixed Viral Infections: Viral Interference, Persistence and Exclusion. PLoS One. 2016 May 26;11(5):e0156110. doi: 10.1371/journal.pone.0156110. eCollection 2016.
  36. Garg R, Patil PK, Singh SV, Sharma S, Gandham RK, Singh AV, Filia G, Singh PK, Jayaraman S, Gupta S, Chaubey KKTiwari R, Saminathan M, Dhama K & Sohal JS. 2015. Comparative Evaluation of Different Test Combinations for Diagnosis of Mycobacterium avium Subspeciesparatuberculosis Infecting Dairy Herds in India, BioMed Research International, 2015: 1-6.
  37. S. Gupta, K.K. Chaubey, S.V. Singh, A.K. Bhatia, N. Kumar, A. Goel, T.K. Sachan, K.D. Rawat, J.S. Sohal and K. Dhama. Immunoreactivity to culture filtrate proteins of Mycobacterium avium Subspecies paratuberculosis in naturally infected goat and sheep sera. Adv. In Animal & Vet. Sci., Vol. 3 (1): 1-7, 2015.
  38. Tarun Sardana, Ran Vir Singh, Shoor Vir Singh, Vishesh Kumar Saxena, Deepak Sharma, Pravin Kumar Singh, Naveen Kumar, Saurabh Gupta, Kundan Kumar Chaubey, Sujata Jayaraman, Ruchi Tiwari, Kuldeep Dhama , Ashok Kumar Bhatia and Jagdip Singh Sohal.2015. Single nucleotide polymorphism of SLC11A1, CARD15, IFNG and TLR2 genes and their association with Mycobacterium avium subspecies paratuberculosis infection in native Indian cattle population. Indian Journal of Biotechnology. 14: 469-475.
  39. Singh SV, Audarya SD, Singh M, Stephen BJ, Chhabra D, Chaubey KK, Gupta S, Sahzad, Pachoori A, Jayaraman S, Aseri GK, Sohal JS, Bhatia AK, Dhama K. 2015. Development of new ‘ Indigenous Dot-ELISA Kit’ as sensitive field based herd screening test for the diagnosis of john’s disease in the domestic buffalo population. Asian Journal of Animal and Veterinary Advances. 11: 1-9.
  40. Singh S.V., Singh P.K., Kumar N., Gupta S., Chaubey K.K., Singh B., Srivastav A., Yadav S., 2015. Evaluation of goat based ‘Indigenous vaccine’ using novel ‘Indian Bison type’ biotype of Mycobacterium avium subspecies paratuberculosis for the control of Bovine Johne’s Disease in endemic herds of native Indian cattle. Indian Journal of Experimental Biology, Vol. 52, pp. 16-24.
  41. Sohal JS, Singh SV, Singh B, Thakur S, Aseri GK, Jain N, Jayaraman S, Yadav P, Khare N, Gupta S, Chaubey KK, Dhama K. 2015. Control of paratuberculosis: opinions and practices. Advances in Animal and Veterinary Sciences. Vol. 3, Issue 3, pp. 156-163.
  42. Sachan T.K., Singh S.V., Virendra Kumar, Gupta S., Chaubey K.K., Sujata Jayaraman, Mukesh Sikarwar, Sunil Dixit, Dhama K. 2015. Chemical and Ultrastructural characteristics of mycobacterial biofilms. Asian Journal of Animal and Veterinary advances. 10(10): 592-622.
  43. Bjorn John Stephen, Mukta Jain, Kuldeep Dhama, S.V. Singh, Manali Datta, Neelam Jain, Sujata Jayaraman, Manju Singh, Mukta Jain, K.K. Chaubey, S. Gupta, G. K. Aseri, Neeraj Khare, Parul Yadav, Kuldeep Dhama, J. S. Sohal. (2015). Nanotechnology Based Therapeutics, Drug Delivery Mechanisms and Vaccination approaches for Countering Mycobacterium avium subspecies paratuberculosis (MAP) Associated Diseases. Asian Journal of Animal and Veterinary Advances, 10: 830-842.
  44. Bjorn John Stephen, S.V. Singh, Manali Datta, Neelam Jain, Sujata Jayaraman, K.K. Chaubey, S. Gupta, Manju Singh,  et al., (2015). Nanotechnological Approaches for the Detection of Mycobacteria with Special References to Mycobacterium avium Subspecies Paratuberculosis (MAP). Asian Journal of Animal and Veterinary Advances, 10: 518-526.
  45. Brajesh Singh, Mukta Jain, Shoor Vir Singh, Kuldeep Dhama, G K Aseri, Neelam Jain, Manali Datta, Neeraj Khare, Parul Yadav, Sujata Jayaraman, Saurabh Gupta, Dr. Jagdip Singh Sohal, Kundan kumar Chaubey. 2015. Plants as Future Source of Anti-Mycobacterial Molecules and Armour for Fighting Drug Resistance. Asian Journal of Animal and Veterinary Advances.10:443-460.
  46. Singh SV, Chaubey KK, Gupta S, Bhatia AK, Jayaraman S, Kumar N, Goel A, Sohal JS, Rawat KD, Stephen BJ, Singh M, Sahzad & Dhama K. 2015. Indian perspective on ‘Diagnosis and control’ of incurable Paratuberculosis major infection of Domestic Livestock and serious Public Health concern globally. 4th Jammu and Kashmir Agriculture Science Congress, 28-30 october, 2015; SKUAST-J, Chatha, Jammu.
  47. Singh SV, Gupta S, Chaubey KK, Sahzad, Stephen BJ, Singh M, Pachoori A, Sohal JS and Bhatia AK. 2015 on “Vaccine and Vaccination approaches for Johne’s disease” in one-day conference on Antimicrobial Resistance in Livestock Health and Production. XIV NAVS Convocation-cum-Conference, 04 Nov, 2015; IVRI, Bareilly.
  48. Rawat K.D., Chaudhary S., Gupta S., Chaubey K.K., Jayaraman S., Kumar N., Sohal J.S., Sachan T.K., Dhama K., Tiwari R. and Singh S.V. 2014. Potential of ‘Goat Based Vaccine’ using ‘India Bison Biotype’ of Mycobacterium avium Subspecies paratuberculosis in Salvaging a Dairy Farm Consisting of High Yielding Holstein Fresian Cows from Devastation and Closure Due to Outbreak of Bovine Johne’s Disease in Northern India. Advances in Animal and Veterinary science. 2: 638-646.
  49. Singh S.V., Kumar N., Sohal J.S., Singh A.V., Singh P.K., Agrawal N.D., Gupta S., Chaubey K.K., Rawat K.D., Deb R., Dhama K., 2014. First mass screening of the human population to estimate the bio-load of Mycobacterium avium subspecies paratuberculosis in North India. Journal of Biological Sciences, Vol. 14, Issue 4, pp. 237-247.
  50. Singh SV, Thakur S, Agarwal ND, Kumar N, Misri J, Gupta S, Chaubey KK, et al. Screening of human population for type 1 diabetes and Mycobacterium avium subspecies paratuberculosis from Chattarpur district of Madhya Pradesh (India). Biomed Res Int. 2014b; 2:612-619.
  51. Singh S.V., Yadav R.K., Gupta V.K., Gupta S., Chaubey,  K.K., Agarwal N.D., Kumar N.,2014. Co-incidence of Bovine Johne’s disease and Bovine Brucellosis in Young Bulls of Murrah Breed in their Native Tract (Rohtak, Haryana, India). Advances in Animal and Veterinary Sciences, 2(IS): 23-25.
  52. Singh S.V., Sohal J.S., Kumar N., Gupta S., Chaubey K.K., Rawat K.D., Chakraborty S., Tiwari R.  and Dhama K., 2014. Recent  approaches  in  diagnosis  and  control  of  mycobacterial  infections  with  special  reference  to  mycobacterium  avium subspecies paratuberculosis. Advances in Animal and Veterinary Sciences, Vol. 2, Special Issue (IS), pp. 1-11.
  53. Barad D.B., Chandel B.S., Dadawala A.I., Chauhan H.C., Kher H.N., Shroff S., Bhagat A.G., Singh S.V., Singh P.K., Singh A.V., Sohal J.S., Gupta S., Chaubey K.K. Chakraborty S., Tiwari R., Deb R., Dhama K., 2014. Incidence of Mycobacterium avium subspecies paratuberculosis in Mehsani and Surti goats of Indian origin using multiple diagnostic tests. Journal of Biological Sciences, Vol. 14, Issue 2, pp. 124-133.
  54. Singh S.V., Gupta S., Chaubey K.K., Rawat K.D., Kumar N., Sohal J.S., Singh S., Tiwari R., Chakraborty S., Dhama K., 2014. Johne’s disease (JD) in a high yielding Holstein Friesian cattle dairy farm in India. Journal of Biological Sciences, Vol. 14, Issue 3, pp. 195-203.
  55. Singh S.V., Gupta S., Chaubey K.K., Kumar N., Rawat K.D., Tiwari R. and Dhama K., 2014. Dose Standardization Studies of ‘Indigenous Vaccine’ for the Control of Mycobacterium Avium Subspecies Paratuberculosis in Naturally Infected Goats. Advances in Animal and Veterinary Sciences, Vol. 2 (IS), pp. 17-22.
  56. Shroff S., Chandel B. S., Dadawala A. I., Gupta S., Chaubey K.K., Singh S.V.,2014. Incidence of Mycobacterium avium subspecies paratuberculosis in Patanwadi sheep. Indian Journal of Small Ruminants, 20(1): 54-58.
  57. Singh S.V.,Gupta, S., Chaubey, K.K., Singh, B., Kumar, A., 2014. Evaluation of microscopy  as a field laboratory test for the diagnosis of Johne’s disease in farm animals. Intas Polivet, 15 (1):41-46.
  58. Pahangchopi D., Singh R.V., Singh S.V., Das P., Sharma D., Sardana T., Kumar N., Chaubey K.K., Gupta S., 2014. Comparative evaluation of two antigens (‘cattle type’ and ‘Indian Bison Type’) of Mycobacterium avium subspecies paratuberculosis for the diagnosis of Bovine Johne’s Disease in a goshala using indigenous ELISA and AGPT.Indian Journal of Experimental Biology. 52(12):1182-5.
  59. Kumar T., Singh R.V., Sharma D., Gupta S., Chaubey K.K., Thakur S., Rawat K.D., Kumar N., Dhama K., Tiwari R., Singh S.V. 2014. Status of Mycobacterium avium subspecies paratuberculosis infection in an Indian goshala housing poorly or unproductive cows suffering with clinical Bovine Johne’s disease. Advances in Animal and Veterinary Sciences, 2(2), doi: http://dx.doi.org/10.14737/journal.aavs/2014/.
  60. Chaudhary, K., Chaubey, K.K., Singh, S.V. and Kumar, N., 2015. Receptor tyrosine kinase regulates Peste des Petits Ruminants virus replication. Acta virologica 59(1):78-83.
  61. Rawat KD, Chaudhary S, Kumar N, Gupta S, Chaubey KK, Singh SV, Dhama K, Verma AK, Deb R. (2014). Economic losses in a commercial dairy farm due to the out-break of Johne’s disease in India. Research Journal for Veterinary Practitioners.2 (5): 73-77.
  62. Kumar A., Singh S.V., Srivastava A.K., Gangwar N.K., Singh P.K., Gupta S., Chaubey K.K. 2014. Comparative evaluation of ‘indigenous’ and commercial vaccines in double challenge model for the control of caprine paratuberculosis in India. Journal of Biological Sciences, Vol. 14, Issue 3, pp. 169-182.
  63. Singh SV, Chaubey KK et al., (2014). Comparision of microscopy and blood-PCR for the diagnosis clinical of Johne’s diseasein domestic ruminant. Iranian Journal of Veterinary Research, 14, 345-349.
  64. Singh SV, Chaubey KK et al., (2014).  Recent  approaches  in  diagnosis  and  control  of  mycobacterial  infections  with  special  reference  to  mycobacterium  avium subspecies paratuberculosis. Advances in Animal and Veterinary Sciences, Vol. 2, Special Issue (IS), pp. 1-11.
  65. Nitin Khandelwal, Gurpreet Kaur, Kundan Kumar Chaubey, Pushpendra Singh, Shalini Sharma, Archana Tiwari, Shoor Vir Singh, Naveen Kumar. 2014. Silver nanoparticles impair Peste des petits ruminants virus replication. Virus Research 190: 1–7
  66. Naveen Kumar, Sunil Maherchandani, Sudhir Kumar Kashyap, Shoor Vir Singh, Shalini Sharma, Kundan Kumar Chaubey and Hinh Ly. Peste Des Petits Ruminants Virus Infection of Small Ruminants: A Comprehensive Review. Viruses 2014, 6, 2287-2327; doi:10.3390/v6062287.
  67. Naveen Kumar, Ashutosh Wadhwa, Kundan K. Chaubey, Shoor V. Singh, Sorabh Gupta, Shalini Sharma, Dinesh K Sharma, Manoj K Singh, A K Mishra. 2014. Isolation and phylogenetic analysis of an orf virus from sheep in Makhdoom, India. Virus Genes. 48:312–319 DOI 10.1007/s11262-013-1025-9
  68. Singh S.V., Singh P.K., Singh A.V., Sohal J.S., Kumar N., Chaubey K.K., Gupta S., Kumar A., Bhatia A.K., Srivastav A.K., Dhama K., 2013. Bio-load and bio-type profiles of Mycobacterium avium subspecies paratuberculosis infection in the farm and farmer’s herds / flocks of domestic livestock: A 28 years study (1985-2013). Transboundary and Emerging Diseases 61 (Suppl. 1) (2014) 43–55.
  69. Singh S.V., Kumar N., Singh S.N., Bhattacharya T., Sohal J.S., Singh P.K., Singh A.V., Singh B., Chaubey K.K., Gupta S., Sharma N., Kumar S., Raghava G.P.S. 2013. Genome Sequence of the “Indian Bison Type” Biotype of Mycobacterium avium subsp. paratuberculosis Strain S5. Journal of Bacteriology: Genome Announcements, Vol. 1, Issue 1, e00005-13.
  70. SinghS.V., Dhama, K., Chaubey, K.K., Kumar, N., Singh,P.K., Sohal,J.S., GuptaS., Singh, A.V., Verma, A.K., Tiwari, R., Mahima, S., Chakraborty and Deb, R. 2013.  Impact of host genetics on susceptibility and resistance to Mycobacterium avium subspecies paratuberculosis infection in domestic ruminants. Pakistan Journal of Biological Sciences Vol. 16, Issue 6, pp. 251-266.
  71. Barad D.B., Chandel B.S., Dadawala A.I., Chauhan H.C., Kher H.N., Shroff S., Bhagat A.G., Singh S.V., Singh P.K., Singh A.V., Sohal J.S., Gupta S., Chaubey K.K..2013. Comparative Potential of Traditional versus Modern Diagnostic Tests in Estimating Incidence of Caprine Johne’s disease. Advances in Animal and Veterinary Sciences. Vol. 1, Issue 1, pp. 35-40.
  72. SinghK., Chandel B.S., Dadawala A.I., Singh S.V., Chauhan H.C., Singh B., Agrawal N.D., Gupta S., Chaubey K.K. 2013. Incidence of Mycobacterium avium subspecies paratuberculosis in Mehsana Breed of Goats from North Gujarat using Multiple Tests. Advances in Animal and Veterinary Sciences. Vol. 1, Issue 1, pp. 28-31.
  73. Singh S.V., Singh P.K., Singh A.V., Gupta S., Chaubey K.K., Singh B., Kumar A., Srivastav A., Singh H., Sohal J.S. 2013. Bio-burden and bio-type profiles of Mycobacterium avium subspecies paratuberculosis infection in the domestic livestock population based on mass screenings using multiple tests. International Journal of Current Research, Vol. 5, Issue 7, pp. 1897-1901.
  74. RaghuvanshiT., Singh S.V., Sharma R.B., Gupta S., Chaubey K.K., Kumar N., Dhama K. 2013. A pilot study on the presence of Mycobacterium avium subspecies paratuberculosis in fresh cheese (paneer) prepared from goat milk of herds endemic for Johne’s disease. Journal of Infection and Molecular Biology, Vol. 1, Issue 3, pp. 46-48.
  75. Singh S.V., Kumar N., Chaubey K.K., Gupta S., Rawat, K.D. 2013. Bio-presence of Mycobacterium avium subspecies paratuberculosis infection in domestic livestock farms. Research Opinions in Animal and Veterinary Sciences, Vol. 3, Issue. 11, pp. 401-406.
  76. Singh S.V., Gupta S., Singh P.K., Sohal J.S., Kumar N., Kumar A., Chaubey K.K., Singh B 2013. Prophylactic study of ‘Indigenous vaccine’ against Johne’s disease in dairy cow and male calf of Hariana breed: Case study. Advances in Animal and Veterinary Sciences, Vol. 1 (1S), pp 23-28. 
  77. Audarya S.D., Chaubey K.K., Gupta S., Kumar N., Yadav D.S., Chhabra D., Sharda R.,Garg U.K., Singh S.V. 2013. Investigation on Bovine Johne’s Disease in an organized dairy farm of Mhow in Madhya Pradesh. International Journal of Current Research, Vol. 5, Issue, 10, pp. 2895-2897.
  78. Singh S.V., Chaubey K.K., Gupta S., Gupta V.K., Agarwal N.D., Kumar N.2013. Co-infection of Mycobacterium avium subspecies paratuberculosis and Brucella melitensis in a Sirohi breed of goats in India. Advances in Animal and Veterinary Sciences, Vol. 1, Issue. 6, pp.188-190.
  79. Kumar N., Chaubey K.K., Singh S.V., Gupta S.,Sharma S.,Sharma D.K., Mishra A.K., Singh M.K. 2014. Phylogenetic analysis of an Indian orf virus isolated from an outbreak in  an organized sheep flock at Makhdoom, India. Virus Genes, 48: 312-319.
  80. Kumar, N., Chaudhary, K., Chaubey, K.K., Singh S.V., Sharma, D.K., Gupta, V.K., Mishra, A.K., and Sharma, S., 2013. Isolation, identification and characterization of a Peste des Petits Ruminants virus from outbreak in Nanakpur India. J. Virological Methods: 189: 388-92.
  81. S.V. Singh, K. Dhama, K.K. Chaubey, N. Kumar, P.K. Singh, J.S. Sohal, S. Gupta, 2012. Impact of host genetics on the control of Mycobacterium avium subspecies paratuberculosis infection in domestic livestock. Lead paper in ISSGPU publication 2012, Chapter 15, Page 255-280 

GENE & GENOME SEQUENCES PUBLISHED IN NCBI:

  1. Submitted Genome Sequence of the 'Indian Bison Type' Biotype of Mycobacterium avium subspecies paratuberculosis Strain 'S5' under genomeA00005-13
  2. Submitted the sequence of the nucleoprotein gene (N) of PPR virus under accession number # KC200262
  3. Submitted the sequence of the Major envelope protein gene, partial cds of Orf virus under accession number # KC992325.
  4. The sequences of the six MAP CFPs genes (MAP 1693c, MAP 2168c, MAP Mod D, MAP 85C, MAP Pep AN and MAP Pep AC) have published in NCBI and Genebank accessioned by following IDs MG753462, MG753463, MG753464, MG753465, MG753466 and MG753467, respectively. 

Chaubey,K.K., Singh,S.V., Gururaj,K., Gupta,R.D., Rathore,A.S., Kumar,N., Gupta,S., Singh,M. and Bhatia,A.K. Mycobacterium avium subsp. paratuberculosis strain S5 peptidyl-prolyl cis-trans isomerase gene, partial cds. GenBank: MG753462.1.

Chaubey,K.K., Singh,S.V., Gururaj,K., Gupta,R.D., Rathore,A.S., Kumar,N., Gupta,S., Singh,M. and Bhatia,A.K. Mycobacterium avium subsp. paratuberculosis strain S5 putative low molecular weight antigen gene, partial cds. GenBank: MG753463.1 

Chaubey,K.K., Singh,S.V., Gururaj,K., Gupta,R.D., Rathore,A.S., Bhatia,A.K., Kumar,N., Gupta,S. and Singh,M. Mycobacterium avium subsp. paratuberculosis strain S5 ModD (modD) gene, partial cds. GenBank: MG753464.1 

Chaubey,K.K., Singh,S.V., Gururaj,K., Gupta,R.D., Rathore,A.S., Bhatia,A.K., Kumar,N., Gupta,S. and Singh,M. Mycobacterium avium subsp. paratuberculosis strain S5 secreted fibronectin-binding protein C antigen 85-C (fbpC) gene, partial cds. GenBank: MG753465.1 

Chaubey,K.K., Singh,S.V., Gururaj,K., Gupta,R.D., Rathore,A.S., Bhatia,A.K., Kumar,N., Gupta,S. and Singh,M. Mycobacterium avium subsp. paratuberculosis strain S5 serine protease PepAN (pepAN) gene, partial cds. GenBank: MG753466.1 

Chaubey,K.K., Singh,S.V., Gururaj,K., Gupta,R.D., Rathore,A.S., Bhatia,A.K., Kumar,N., Gupta,S. and Singh,M. Mycobacterium avium subsp. paratuberculosis strain S5 serine protease PepAC (pepAC) gene, partial cds. GenBank: MG753467.1

 
5. The sequences of the MAP2191c (MAP Mce) gene has been published in NCBI and Genebank accessioned by IDs MG754208. 
Hemati,Z., Derakhshandeh,A., Haghkhah,M., Singh,S.V., Chaubey,K.K., Gururaj,K., Gupta,S., Singh,M., Gupta,R.D. and Rathore,
A.S. Mycobacterium avium subsp. paratuberculosis strain S5 mammalian cell entry protein (mce) gene, partial cds. GeneBank: MG754208.1  

ABSTRACT PUBLISHED:

Abstract published to 14th ICP to be held on 4-8 June 2018 at Riviera Maya, Mexico 

Oral:

1. Kundan Kumar Chaubey, ShoorVir Singh, Rinkoo Devi Gupta, KirtiDua, Shukriti Sharma, NiranjanaSahoo. Recombinant secretary proteins based ‘Cocktail ELISA’ to differentiate between infected and vaccinated bovines against Mycobacterium avium subspecies paratuberculosis. 

Poster: 

2. Zahra Hemati, Kundan Kumar Chaubey, AbdollahDerakhshandeh, MasoudHaghkhah, Abhishek Singh Rathore, ShoorVir Singh. Novel recombinant Mce-truncated protein antigens for diagnosis and control of paraTB infection in domestic livestock. 

1st satellite conference, IAB, UK (12 October, 2016). 

3. Kundan Kumar Chaubey, Rinkoo Devi Gupta, Saurabh Gupta, Shoor Vir Singh, Ashok Kumar Bhatia, Sujata Jayaraman, Naveen Kumar, Abhishek Singh Rathore, Sahzad, Bjorn John Stephen, Manju Singh, Mukta Jain, Kuldeep Dhama, Jagdip Singh Sohal. 2016. Evaluation of recombinant CFPs based cocktail ELISA vis a vis Indigenous ELISA using semi-purified protoplasmic antigen to differentiate infected from vaccinated population of domestic livestock. 

4. Kundan Kumar Chaubey, Sahzad, Shoor Vir Singh, Premanshu Dandapat, Saurabh Gupta, Bjorn John Stephen, Manju Singh,Jagdip Singh Sohal, Mukta Jain, Gajendra Kumar Aseri, Manali Dutta, Neelam Jain, Parul Yadav, Jitendra Chauhan, Kuldeep Dhama. 2016. Bio-incidence of Mycobacterium avium subspecies paratuberculosis infection in suspected domestic livestock population in India using multiple tests.

In the 13th International Colloquium on Paratuberculosis (ICP), Nantes, France (20-24, June, 2016)- 1 Oral and 7 Posters 

Oral:

5. Kundan Kumar Chaubey, Saurabh Gupta, Ashok Kumar Bhatia, Jagdip Singh Sohal, Sujatha Jayaraman, Bjorn John Stephan, Manju Singh, Mukta Jain, Krishna Dutta Rawat, Naveen Kumar, Shoor Vir Singh. Recombinant secretory proteins based diagnostic test to investigate differential immune response to infection and vaccination against Mycobacterium avium subspecies paratuberculosis in domestic livestock. 

Posters:

6. Kundan Kumar Chaubey, Saurabh Gupta, Jagdip Singh Sohal, Sujatha Jayaraman, Bjorn John Stephan, Manju Singh, Mukta Jain, Naveen Kumar, Shoor Vir Singh. Comparison of ‘Indigenous ELISA kit’ with ‘Ethanol Vortex ELISA kit (USA)’ for the detection of Mycobacterium avium paratuberculosis infection in cattle population in search of a ‘globally relevant kit’. 

7. Satish kumar, Subodh kumar, Ran Vir Singh, Amit Kumar, Saurabh Gupta, Kundan Kumar Chaubey, Naveen Kumar, Shoor Vir Singh. Identification of polymorphism in clec7a gene and their association with occurrence of paratuberculosis in cattle.

8. Saurabh Gupta, Kundan Kumar Chaubey, A.K. Bhatia, Shoor Vir Singh, Naveen Kumar, Sahzad, Manju Singh, Bjorn John Stephen, Sujata Jayaraman, Mukta Jain and Jagdip Singh Sohal. Identification of Immuno-reactive secretory proteins of Mycobacterium avium subspecies paratuberculosis vaccine strain ‘S 5’ with a diagnostic potential in early infection. 

9. Saurabh Gupta, Shoor Vir Singh, Kundan Kumar Chaubey, Manju Singh, Bjorn John Stephan, Sahzad, B. Lahiri, Prabhat Agarwal, Ashok Kumar Tyagi, Jyoti Misri and Jagdip Singh Sohal. Bio-load of Mycobacterium avium subspecies paratuberculosis infection in human patients suffering with thyroid disorder in Agra region of North India using ‘Indigenous ELISA and Real Time_PCR. 

10. Bjorn John Stephan, Manju Singh, Saurabh Gupta, Kundan Kumar Chaubey, Sahzad, Shoor Vir Singh, Sujata Jayaraman, Mukta Jain, Gajendra Kumar Aseri, Manali Datta, Neelam Jain, Parul Yadav, Neeraj Khare, Kuldeep Dhama, Jagdip Singh Sohal. Identification of Mycobacterium avium subspecies paratuberculosis in milk products (paneer or fresh cheese) from local dairies in Farah, Mathura and Agra employing six diagnostic tests. 

11. Tarun Kumar Sachan, Kundan Kumar Chaubey, Saurabh Gupta, Krishna Dutta Rawat, Naveen Kumar, Jagdip Singh Sohal, Shivalingappa Yamanappa Mukartal, Kuldeep Dhama, Shoor Vir Singh. DEVELOPMENT AND ULTRASTRUCTURAL ANALYSIS OF MYCOBACTERIUM AVIUM PARATUBERCULOSIS BIOFILM OF DIFFERENT CLINICAL ISOLATES.

 12. J S Sohal, Sujata Jayaraman, K K Chaubey, Mukta Jain, Rinkoo D Gupta, G K Aseri, S V Singh, Saurabh Gupta, Manali Datta, Neelam Jain, Parul Yadav, Neeraj Khare. Optimization of marker assay for diagnosing the immune response due to vaccination and natural infection. 

13. Sujata Jayaraman, K K Chaubey, Rinkoo D Gupta, Manju Singh, Mukta Jain, Gaurabh Gupta, Saurabh Gupta, S V Singh, G K Aseri, Manali Datta, Neelam Jain, Parul Yadav, Neeraj Khare, J S Sohal. Performance of serological assay using specific proteins of Mycobacterium avium subspecies paratuberculosis for diagnosis of Large Ruminants. 

14. Sujata Jayaraman, K K Chaubey, Rinkoo D Gupta, Manju Singh, Mukta Jain, Gaurabh Gupta, , Saurabh Gupta, S V Singh, G K Aseri, Manali Datta, Neelam Jain, Parul Yadav, Neeraj Khare, J S Sohal. A new Serology test for diagnosis of small ruminant paratuberculosis using specific antigens

15. Mukta Jain, K K Chaubey,Saurabh Gupta, Manju Singh, S V Singh, Manjula Singh, Sachin Kumar Verma, G K Aseri, Manali Datta, Neelam Jain, Parul Yadav, Neeraj Khare, Bharti Malhotra, Sumit Goyal, J S Sohal. Investigation of Paratuberculosis in domestic ruminant farms of Western India (Rajasthan) under different management practices: A first Report  

12th IGA conference, Antalya, Turkey 2016 

16. Saurabh Gupta, Kundan Kumar Chaubey, Shoor Vir Singh, Sujata Jayaraman, Manju Singh, Bjorn John Stephan, Krishna Dutta Rawat, Anjali Pachoori, Jagdip Singh Sohal ,A.K. Bhatia and Kuldeep Dhama. Revisited Immuno-reactivity between native semi-purified protoplasmic (caprine) versus commercial purified protoplasmic (bovine) antigens for the screening of goatherds endemic for Johne’s disease. 

17. S.Y.Mukartal, D.Rathannama, Manju Singh, Bjorn J. Stephan, Saurabh Gupta Kundan Kumar Chaubey, Sujata Jayaraman, Sahzad, Anjali Pachoori, Kuldeep Dhama, ShoorVir Singh. High bioincidence of ovine johne’s disease in the mandaya breed in south india using multiple diagnostic tests and biotyping of mycobacterium avium subspecies paratuberculosis infection. 

18. Shoor Vir Singh, Saurabh Gupta, Kundan Kumar Chaubey, Krishna Dutt Rawat, Naveen Kumar, Sujata Jayaraman, Jagdip Singh Sohal, Kuldeep Dhama. Therapeutic efficacy’ of ‘Indigenous vaccine’ in Jakhrana goat breed for the treatment of Johne’s Disease. 

In the 12th International Colloquium on Paratuberculosis (ICP), Parma, Italy (22-26, June, 2014)- 8 Poster and 1 Oral 

19. S.V. Singh, J.S. Sohal, P.K. Singh, A. V. Singh, N. Kumar, A. K. Bhatia, A. K. Srivastava, S. Gupta, K.K.Chaubey, S. Thakur, K.D. Rawat, B. Singh, K. Dhama, R. Tiwari, R. Deb, P. K. Agarwal, 2014. A decade (2002-2013) of molecular epidemiology (biotype profiles) and inter-species sharing of Mycobacterium avium subspecies paratuberculosis  in domestic and wild ruminants (blue bulls, deer, bison), other animals, primates and human beings in India   (39137) 

20. S.V. Singh, N. Kumar, A.V. Singh, P.K. Singh, J.S. Sohal, N.D. Agarwal, S. Gupta, K.K. Chaubey, S. Thakur, K.D. Rawat, B. Singh, K.Dhama, R. Tiwari, R. Deb, P.K. Agarwal, A. Kumar, 2014. Trends in bio-load of Mycobacterium avium subspecies paratuberculosis infection in human  population with special reference to individuals suffering with chronic ailments from North India in last decade (2004 to 2014)   (39114) 

21.S.V. Singh, K.D. Rawat, S. Singh, N. Kumar, S. Gupta, K.K. Chaubey, P. Birthal, S. Thakur.Economic losses due to the breakout of clinical Johne’s disease in high yielding Holstein Friesian cows located in a dairy farm at Alwar district of Rajasthan, India (38999) 

22. S.V. Singh, P.K. Singh, A.V. Singh, J.S. Sohal, S. Gupta, N. Kumar, A.K. Bhatiya, A.K. Srivastav, S.N. Singh, T. Bhattacharya, B.S. Chandel, A. Dadawala, B. Singh, N. Swain, A. Kumar, M.K. Singh, S. Bhushan, N. Ramachandran, K.K. Chaubey, K.D. Rawat, D. Swarup, M.C.Sharma, N.P.Singh, S.K.Agarwal. Status and potential of ‘Indigenous Vaccine’ in the therapeutic management and control of Johne’s disease in the endemically infected population of goats, sheep, cattle and buffaloes in India  (39001) 

23.S. Gupta, S.V. Singh, A.K. Bhatia, N. Kumar, A. Kumar, K.K. Chaubey, K.D. Rawat.Secretory proteins profile of Mycobacterium avium subspecies paratuberculosis with different growth patterns   (39199) 

24.S.V. Singh, P.K. Singh, A.V. Singh, J.S. Sohal, N. Kumar, A.K. Bhatia, R.B. Sharma, S. Gupta, K.K. Chaubey, S. Thakur. Bio-presence of Mycobacterium avium subspecies paratuberculosis in milk and milk products in India and its’ Public Health Significance. 12th ICP, Parma, Italy (22-26, June, 2014) 

25.Shivam Chaturvedi, Shoor Vir Singh, A K Srivastava, Naveen Kumar, Rajendra Singh, Saurabh Gupta, Kundan Kumar Chaubey. Status of diagnostic efficacy of different tests for Mycobacterium avium subspecies paratuberculosis infection in endemically infected goats with respect to the pathological lesions. 12th ICP, Parma, Italy (22-26, June, 2014) 

26. S.V.Singh, P.K.Singh, A.V.Singh, J.S.Sohal, Naveen Kumar, A.K.Bhatia, S. Gupta, K.K.Chaubey, H.A.Tiwari. Status and dynamics of Mycobacterium avium subsp. paratuberculosis bioload in the biotic (domestic livestock species, wild ruminants, other animals, primates and human beings) environment of country: A fact sheet of 28 years. 12th ICP, Parma, Italy (22-26, June, 2014) 

27. S.V. Singh, P.K. Singh, A.V. Singh, J.S. Sohal, S. Gupta, N. Kumar, A.K. Bhatiya, A.K. Srivastav, S.N. Singh, T. Bhattacharya, B.S. Chandel, A. Dadawala, B. Singh, N. Swain, M.K. Singh, Saket Bhushan, K.K. Chaubey, K.D. Rawat, D. Swarup, M.C. Sharma, N.P. Singh, S.K. Agarwal. Status and potential of ‘Indigenous Vaccine’ in the therapeutic management and control of Johne’s disease in the endemically infected population of goats, sheep, cattle and buffaloes in India. 

12th ICP, Parma, Italy (22-26, June, 2014) 

Poster in National Conference: 

1. Zahra Hemati, Abdollah Derakhshandeh, Masoud Haghkhah, Kundan Kumar Chaubey, Saurabh Gupta, Manju Singh, ShoorVir Singh. Recombinant mammalian cell entry (mce) protein of Mycobacterium avium subspecies paratuberculosis based diagnosis for detection of paratuberculosis infection at National seminar on Improvement of small ruminant production system for livelihood security and Annual conference of ISSGPU, 9-10, March, 2017, CSWRI, Avikanagar, Rajasthan

2. Kundan Kumar Chaubey, Saurabh Gupta, Ashok Kumar Bhatia, Anjana Goel, Krishna Dutta Rawat, Tarun Kumar Sachan, Vibhuti Sharma, Naveen Kumar and Shoor Vir Singh. Development of a recombinant secretary protein based ELISA for differentiation of Mycobacterium avium subspeciesparatuberculosis infected and vaccinated animals (2014) IAVMI-2014, XXVIII Annual convention of IAVMI & International conference held at DUVASU & GLA University, Mathura proceedings S5:P15;62 

3. Saurabh Gupta, A.K. Bhatia, Shoor Vir Singh, Anjana Goel, Naveen Kumar,  Kundan Kumar Chaubey, Tarun Kumar Sachan and Krishan Dutta Rawat. Proteome analysis of secreted proteins from Mycobacterium avium subspecies paratuberculosis for the diagnosis of Johne’s disease. (2014) IAVMI-2014, XXVIII Annual convention of IAVMI & International conference held at DUVASU & GLA University, Mathura proceedings S11:P23;190

 4. S.V. Singh, N. Kumar, S.N. Singh, T. Bhattacharyya, K.K. Chaubey, P.K. Singh, A.V. Singh, J.S. Sohal, 2012. Prospects of Control and Eradication of Johne's Disease in India. RDHM-C01, ISSGPU-2012, pp. 149. 

5. S.V. Singh, K.K. Chaubey, N. Kumar, P.K. Singh, J.S. Sohal, S. Gupta, 2012. Impact of host genetics eterinary on the control of Mycobacterium avium subspecies paratuberculosis infection in domestic  livestock. ISSGPU-2012 pp.76. 

6. S.V. Singh, P.K. Singh, A.V. Singh, S. Gupta, K.K. Chaubey, B. Singh, A. Kumar, A. Srivastav, H. Singh, J.S. Sohal. Multiple tests based screening of domestic livestock population from different agro-climatic regions to estimate bio-burden and bio-type profiles of Mycobacterium avium subspecies paratuberculosis infection in 2010-2011. RDHM-C25, ISSGPU-2012, p.162. 

7. S.V. Singh, P.K. Singh, S. Gupta, K.K. Chaubey, B. Singh, A. Kumar, A.V. Singh, N. Kumar. Comparative evaluation of microscopy, as a 'field laboratory test' vis a vis blood-PCR for the diagnosis and estimation of prevalence of Mycobacterium avium subspecies paratuberculosis infection in domestic ruminant population suffering with clinical Johne's disease in India. RDHM-C24, ISSGPU-2012, pp.161. 

8. S.V. Singh,  A.V. Singh, P. K. Singh, J. S. Sohal, N. Kumar, H. A. Tiwari, K. K. Chaubey, S. Gupta Incidence of Mycobacterium avium subspecies paratuberculosis infection in the Indian domestic livestock population. RDHM-C23, ISSGPU-2012, pp.161. 

9. S.V. Singh, N. Kumar, S.N. Singh, T. Bhattacharyya, K.K. Chaubey, P.K. Singh, A.V. Singh, J.S. Sohal, 2012. Prospects of Control and Eradication of Johne's Disease in India. RDHM-C01, ISSGPU-2012, pp. 149. 

10. Bio-burden of Mycobacterium avium subspecies paratuberculosis in the biotic (animals, primates and human beings) and abiotic (oil and water resources, raw and pasteurized milk and milk products) environment and strategies for control  in ISSGPU-2012 

11.  S.V. Singh, K.K. Chaubey, N. Kumar, P.K. Singh, J.S. Sohal, S. Gupta, 2012. Impact of host genetics on the control of Mycobacterium avium subspecies paratuberculosis infection in domestic  livestock. ISSGPU-2012 pp.76. 

12.  “Isolation, identification and characterization of a Peste des Petits Ruminants virus from outbreak occurred in Nanakpur, India” in ISSGPU-2012 

13. “A host cell kinase that regulates Peste des Petits Ruminants Virus replication” in ISSGPU-2012 

14.Isolation and identification of a goatpox virus from an outbreak occurred in Kanker District of Chhatissgarh in ISSGPU-2012. 

15. Receptor tyrosine kinase signaling regulates Peste des Petits Ruminants virus replication in XXI National conference of Indian Virological Society on “Immunobiology and Management of Viral Diseases in 21 century (IMVD-2012)” held at Indian Veterinary Research Institute, Mukteshwar 263138 (UKD), India from 8-10th November 2012. 

POSTER PRESENTATION 

1. First prize obtained for Poster presentation on “Isolation, identification and characterization of a Peste des Petits Ruminants virus from outbreak occurred in Nanakpur, India” at Central Institute for Research on Goats in ISSGPU-2012.

2. Poster presented on “Transgenic plant” at Bundelkhand University in KEPLER-2006. 

3. Poster presented on “Screening of Lactobacillus Isolates for Antimicrobial Agent” at Gwalior Cancer Institute in National Symposium on Recent trends in molecular biology (NSRTMB) -2006. 

4. Poster presented on “Screening Of Antimicrobial activity of Lactobacillus in food preservation” at Bundelkhand University in National Seminar On Women Entrepreneurial Development -2006. 

CONFERENCE AND SEMINAR

1.Conference and seminar attended at M.L.B. Medical College, Jhansi (U.P.) in Microcon, 2006.

2. Conference and seminar attended at Calcutta University in PTCA, 2010.

3. Seminar attended at SINP, Kolkata on “Indian Proteomics: User’s perspective”2010. 

DEBATE 

1. Debate on “Genetically Modified Organism” ” at Gwalior Cancer Institute in National Symposium on Recent Trends in Molecular Biology (NSRTMB)-2006 

 REFERENCE 

Dr. Shoorvir Singh

Professor & Head

Department of Biotechnology, GLA University

Chaumuhan, Mathura, UP, India 281406                                                                                                            

Phone No: 09719072856; 09412826674

Email: shoorvir.singh@gmail.com; shoorvir_singh@rediffmail.com

 

Prof. Ashok Kumar Bhatia

Professor

Department of Biotechnology

GLA University, Mathura, UP 281406

Phone No: 08869837271

 Email:  ak.bhatiya@gla.ac.in, akbhatia33@yahoo.com 

Dr. Kuldeep Dhama

Principal Scientist

Pathology Division

Indian Veterinary Research Institute (IVRI),

zatnagar, Bareilly, Uttar Pradesh, India

Email: kdhama@rediffmail.com  

Dr. Naveen Kumar

Senior scientist                                                                                                               

Animal Health Division                                                                                                                                       

Central Institute for Research on Goats

Makhdoom, Farah, Mathura, U.P 281122

Phone No: 09992117990

Email: naveenkumar.icar@gmail.com 

I declare that the above information is true to the best of my knowledge and belief.                                                                                                                                                                                                                                                                      

Date   : 

Place :   Mathura                        (Kundan Kumar Chaubey)

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